摘要
观察了醋酸铅１，５，１０，５０，１００μｍｏｌ／Ｌ对培养的ＬＬＣ－ＰＫ１细胞存活率、细胞内钾离子和细胞上清液中ＬＤＨ和γ－ＧＴ的影响。结果５０μｍｏｌ／Ｌ和１００μｍｏｌ／Ｌ铅作用３２小时细胞的存活率已明显减少；５μｍｏｌ／Ｌ铅处理１小时，细胞上清液中ＬＤＨ明显升高，并在１～１０μｍｏｌ／Ｌ范围内存在明显的时间－效应、剂量－效应关系；５０μｍｏｌ／Ｌ铅处理半小时，就出现细胞内钾离子明显减少；１～１００μｍｏｌ／Ｌ铅处理４小时和８小时，γ－ＧＴ已明显增加，并存在剂量－效应关系。加锌２０μｍｏｌ／Ｌ可使细胞的存活率增加，细胞内钾离子和γ－ＧＴ、ＬＤＨ的漏出明显减少。上述结果说明，醋酸铅作用于ＬＬＣ－ｐＫ１细胞，首先引起细胞膜对钾离子通透性的改变，其次为ＬＤＨ，当明显损伤细胞膜时可使膜结合酶γ－ＧＴ脱落，上述指标在一定范围内存在时间－效应和剂量－效应关系。２０μｍｏｌ／Ｌ锌在一定程度上可保护细胞膜，拮抗铅对肾小管细胞ＬＬＣ－ｐＫ１的损伤作用。
In this study ,changes of cellular vitalities ,cellular K +contents,supernatant LDH and r-GT activity of cullured LLC-PK1 cell induced by 1,5,10,50 ,100μmol/L acetate lead (Pb) were investigeted ,the results showed that the cellular vitality decreased signiffcantly after 32 hours of 50and 100μmol/L.Pb treatment;the supernatant LDH actvity increased significantly after 1 hour of 5μmol/L Pb treatment ,and showd a time-effect and dose-response relationship between 1 and 10μmol/L Pb ,after half and hor of 50 μmol/L Pb treatment ,the cellular K+ contents deceased significantly;activties of r-GT increased sigficantly after 4 and 8 hour of Pb treatment and showed a dos-response relationship.addition of 20 μmol/L sulfate Zinc (Zn) showed an effect to make the significant increases of cellular vitalities and the significant decereases of leakage of cellular K+,LDH and r-GT,these results suggest that Pb act on the menbrane of the LLC-Pkl cells and make the changes of membrane K+ premeability firstly ,followed by the changes of LDH activities ,while r-GT,a membrane combine enzyme, detachede only when the membrane is heavily damaged.the parmeters showed a time-effect and dose-response relationship among certainranges.20μmol/L Zn have some protective effect on membrane damage and against the toxicity effect of Pb to kidney tubules.
出处
《卫生毒理学杂志》
CSCD
1995年第2期73-76,共4页
Journal of Health Toxicology
关键词
铅
肾毒性
细胞毒性
锌
工业毒理
LLC-PK1 cells
acetate lead
sulfate Zinc
lactate dehydrogenase
K￣+ions
r-glutamyltransferase
