摘要
观察了０．０５～１０００ｍｍｏｌ／Ｌ氟化钠与０．００１～２０ｍｍｏｌ／Ｌ亚硒酸钠单独或联合对大鼠离体肝匀浆和肝细胞碱性磷酸酶（ＡＫＰ）和谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性以及肝细胞内活性氧代谢全过程的影响。结果表明，硒可消除或减轻氟对ＡＫＰ的抑制作用；氟未改变硒对ＧＳＨ－Ｐｘ的激活作用；硒对肝细胞内活性氧代谢的诸多有利作用，如羟自由基（ＯＨ＊）产生量减少、脂质过氧化物（ＬＰＯ）含量下降等，并未因氟的共有而减弱。
The effects of NaF (0. 05～ 1000 mmol/L)and /or Na2SeO3·5H2O(0. 001～20mmol/L)on the activities of alkaline phosphatase (AKP)and glutathione peroxidase(GSH-Px),and on the metabolism of oxygen free-radicals in the rat liver and in the cultured hepatocytes were explored in vitro. The results showed that selenium could eliminate or decrease the inhibition effect of fluoride on the AKP activity. Fluoride could not change the enhancing effect of selenium on the GSH-Px activity.Fluoride could not change either the beneficial effects of selenium on the metabolisms of oxygen free-radicals, such as the decreases of hydroxyl ftee radical(OH*) output and lipoperoxide(LPO) content in the cultured hepatocytes.
出处
《卫生研究》
CAS
CSCD
北大核心
1995年第3期148-151,共4页
Journal of Hygiene Research
基金
国家自然科学基金
关键词
氟
硒
肝酶
自由基
病理
大鼠
fluoride
selenium
liver enzyme
oxygen free-radicals
cultured hepatocyte
