摘要
提出了表面活性剂增敏的碱性降解荧光法测定四环索新方法，四环素在０．５ｍｏｌ／ＬＮａＯＨ溶液中反应，生成强荧光降解物，加入十六烷基三甲基溴化铵后。四环素荧光强度增强１５０倍，浓度在９．０×１０￣（－９）～１．０×１０￣（－６）ｍｏｌ／Ｌ范围内荧光强度与四环素含量成正比。本法成功地用于尿液和血清中四环素的回收测定。
A New fluorimetric method, involving alkaline degradation and surfactantenhancement,is proposed for the determination of tetracycline. Tetracycline reacts with 0.3 mol/L NaOH and produces strong fluorescent species. Its fluorexcence intensity enhance about 1 5O-fold after degradation and adding CTMAB. Over the range of 9.0×10￣(-9)～1. 0×1O￣(-6)mol/L,thefluorescence intensity of the degradation product is proportional to the concentration of tetracy-cline. The deterniination of tetracvcline in urine and serum is successful with satisfactorv recov-ery.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1995年第2期238-242,共5页
Journal of Xiamen University：Natural Science
基金
厦门大学育苗基金
