摘要
采用聚丙烯酰胺凝胶电泳方法，对豢养条件下野牦牛的血清α－淀粉酶（α－Ａｍ）进行分离和理化性质检测，并与家牦牛比较．结果表明，分别可分离出野、家牦牛α－Ａｍ同工酶的７条和８条电泳区带，二者相比，野牦牛α－Ａｍ同工酶的ｐＨ作用区限较宽但热稳定性较低，家牦牛Ａｍ－３的活性和相对百分含量较高且稳定。野、家牦牛α－Ａｍ同工酶的差异本质上是因其所处生态条件不同而在淀粉类代谢类型上表现出种属特异性，对家牦牛的品种培育研究是颇有用的种质。
By use of polyacrylamide-gel electrophoresis,blood samples from wild anddomestic yak are studied for α-Amylase(α-Am)isozymes.It has been found that there are 7bands in wild yak for α-Am isozyme,but 8 bands in domestic yak.Active boundary of pH in wildyak are wider than that in domestic yak and sensibility to temperature in wild yak are higher thanthat in domestic yak. Activity and relative content of Am-3 for domestic yak are significantlyhigher than that for wild yak.The difference of α-Am isozymes between wild and domestic yakprovides germplasm material for yak breeding selection.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1995年第5期786-790,共5页
Journal of Xiamen University：Natural Science
基金
"八五"农业部畜牧重点科研资助项目。
