摘要
本研究采用体外模拟体内胃肠道环境某些条件用透析方法研究了魔芋精粉与Ｐｂ（２＋）、Ｃｄ（２＋）、Ｆｅ（２＋）、Ｚｎ（２＋）、Ｃａ（２＋）的结合。并探讨了加热处理、ｐＨ改变及环境共存离子的竞争作用对结合的影响。结果表明，魔芋精粉与五种离子的结合具ｐＨ依赖性，ｐＨ５～５．６时发生不同程度的结合，ｐＨ１．５时无结合，ｐＨ６．５时随离子浓度增加魔芋精粉与Ｐｂ（２＋）、Ｃｄ（２＋）的结合降低，与Ｃａ（２＋）的结合增加，与Ｚｎ（２＋）的结合不变，与Ｆｅ（２＋）不结合。结合率Ｚｎ＞Ｐｂ、Ｃｄ＞Ｃａ＞Ｆｅ，五种离子共存时Ｆｅ、Ｃａ与魔芋精粉的结合有竞争性。ｃａ自魔芋精粉中被Ｆｅ置换出，Ｐｂ、Ｃｄ、Ｃａ、Ｚｎ的结合明显降低，对Ｆｅ的结合明显增加。对实验结果进行Ｓｃａｔｃｈａｒｄ作图表明魔芋精粉具有与Ｐｂ（２＋）、Ｃｄ（２＋）特异结合位点。
The binding of Pb,Fe,Zn,Cd,Ca,to refined konjic powder(RKP)was determined in vitro mesans of dialysis in the simulated gastrointestinal conditions,the results showed metal binding to RKP to be pH dependent and to different extent at ph 5.07 and 6.51 except Fe.The affinity of RKP to metal ion was found to be Ze>Pb and Cd>Ca>Fe.The results also suggested that there might be competitive binding between Fe and Ca under the presences of 5 metal ions.The binding of Pb,Cd,and Zn to PKP was not affected by other coexistent ions,Heat treatment could decrease the banding of Pb,Cd,Ca,and Zn but incresase the binding of Fe to RKP.Scatchard plots indicsated that there were special binding sites for and Ca in RKP.
出处
《现代预防医学》
CAS
1995年第4期195-197,共3页
Modern Preventive Medicine
基金
国家自然科学基金
