摘要
以旷场试验法测定动物在急、慢性躯体性和心理性应激时的行为变化，以快速断头冷冻匀浆法，用ＨＩＴＡＣＨＩ－８３５氨基酸分析仪测定应激各期视皮层、海马、下丘脑、小脑谷氨酸含量。并对正常动物经侧脑室微量注射Ｌ－ＡＰ４后观察行为变化。结果显示，急性应激期动物行为活动增加，慢性应激期减少：应激时，大鼠部分脑区的谷氨酸含量与对照组相比在不同时期呈显著性差异；侧脑室微量注射Ｌ－ＡＰ４提示行为活动减弱可能与脑内Ｇｌｕ系统的活动有关。
Glutamate(Glu)levels of the visual cortex(VC) ,hippocampus (Hi),hypothalamus(Hy)and cerebellum(Ce)during acute and chronic,and physical and psychological stress in rats were measured.Open-field behaviors (OFBs)during stress and after intracerebroventricularly(icv,lateral ventricle) administered L-AP4(10-3M/L,5ul)or saline(5ul)were recorded with TV camera The results showed that Glu levels of Hi and Hy in acute stress group and that of VC in chronic stress groups were higher and that of Ce was lower than that of the control (P<0.05),and there was a significant difference in Glu level of Hi between physical and psychological stress.OFBs were markedly reduced during chronic stress.It was similar to chronic stressed animals that OFBs in non-stress rats were reduced significantly in the 5min following icv administration of L-AP4.These suggested that stressinduced OFBs change was connected with Glu system in CNS.
出处
《心理学报》
CSSCI
CSCD
北大核心
1995年第4期422-427,共6页
Acta Psychologica Sinica
基金
全军医药卫生科研基金
