摘要
本文介绍了用TLC和HPLC2种方法对硫酸长春地辛原料药进行纯度检验的实验。TLC方法采用硅胶GF_(254)为固定相,乙醚-甲醇-甲胺(20:4:1)为展开剂,在紫外灯254nm下检视。HPLC法采用C_(18)化学键合硅胶为固定相,以0.02mol/L磷酸二氢钾溶液(pH6.6)-甲醇(20:80)为流动相,在267nm波长下检测。结果表明,经优化条件后的TLC和HPLC2种方法均可简便准确地检测硫酸长春地辛原料药中杂质含量,二者结果一致。
TLC and HPLC methods were developed for the determination of the purity in vindesine sulfate,TLC method was,carried out by using silica gel F254 and ether-methanol-methylamine(20:4:1)as the mobile phase and UV detection at 254 nm. In HPLC analysis,analytical column was packed with C18-bonded silica gel,a solution of methanol and 0.02mol/L potassium dihydrogen phosphate (pH6. 6)(80:20)was employed as the mobile phase,the 267nm UV detector was selected.TLC and HPLC can determine the the purity in vindesine sulfate simply and pricisely under the optimized conditions.The results of the two methods are identical.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1995年第6期8-10,共3页
Chinese Journal of Pharmaceutical Analysis