摘要
采用１２５Ｉ－ＵｄＲ同位素释放试验，观察利巴韦林（三氮唑核苷，ＲＴＣ）在体外对小鼠脾细胞自然杀伤（Ｎｋ）活性的影响。结果表明，ＲＴＣ的剂量与ＮＫ活性呈负相关，在５ｕｍｏｌ·Ｌ－１时ＲＴＣ即可显著抑制ＮＫ活性。ＲＴＣ与地塞米松合用对ＮＫ活性的抑制有相加作用，而ＲＴＣ与γ干扰素合用则互为拮抗作用。此外，还发现ＲＴＣ能明显降低刀豆蛋白Ａ诱生的小鼠脾细胞干扰素及白细胞介素－２的产量，推测ＲＴＣ对ＮＫ活性抑制的部分机理是阻碍了这些淋巴因子的生成。
The effects of ribvirin(1-β
-D-ribofuranosy 1-1,2,4-triazole-3-carboxamide,RTC)on natural
killer(NK)activities of murine spleen cells in vitro were studied
by125I-UdR isotoperelease assay.The results showed that RTC at the
concentration of 5 umol·L-1 significantlysuppressed NK
activities(P<0.01),and a negative correlation between NK activities
and RTCdosage was obtained.It also indicated that there was additive
effect between the actions of RTC anddexamethasone(Dex,1
umol·L-1),and antagonism between the actions of RTC and interferon-
γ(100 U·ml-1).In addition,RTC at the concentration of 0.5 umol·L-1
was found to be enough toreduce the product of interferon(P<0.01)and
interieukin-2(P<0.05)induced by Con A in murinespleen cells,and a
negative dcoe-effect relationship was obeerved.Thus,one of the
mechanisms ofRTC in inhibiting NK activities seems to suppress the
production of these lymphokines.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第6期417-421,共5页
Acta Pharmaceutica Sinica
关键词
利巴韦林
自然杀伤活性
干扰素
白细胞介素-2
脾
Ribavirin
Natural
killer(NK)activity
Interferon
Interleukin-2
Dexamethasone
