摘要
研究循环免疫复合物(CIC)对于疾病的诊断、疗效判断和了解疾病的发展有重要意义。许多研究表明在麻风病人血清中有CIC的存在,且某些研究提示可能是ENL反应时组织损伤的原因。但至今尚未见到有关麻风病人血中麻风杆菌特异的酚糖脂抗原(PGL1)与其相应抗体(Ab)形成的特异免疫复合物(PGL1-Ab)的研究及其检测方法的报道。
In this article, we first report the development of a new test for detecting specific circulating immune complexes (SCIC) in the sera of leprosy patients: This test was named monoclonal antibody specific binding assay (McAb/SBA). We screened for SCIC (PGLMgG, PGLHgM) in 200 serum batches from 140 leprosy patients, 20 tuberculosis patients and 40 nor mal controls, and compared the McAb/SBA with PGL1/ELISA, The results indicated that:1) McAb/ SBA was highly sensitive (90%) and specific (95%). Its Youden's Index was 90. Except for its specificity of 95%, the sensitivity (85%) and Youden's Ind-ex (85) of the PGL1/ELISA test were lower than those of McAB/SBA; 2) The positive rate (34/40) in paucibacillary patients using McAb/SBA was higher than that (28/40) in PGL1/ELISA; 3) The increase and decline of MOD values in McAb/SBA were associated with BI. McAb/SBA is a new method for detecting SCIC (PGL1-IgG, PGL1-IgM) in the sera of leprosy patients, and it is more sensitive than PGL1-ELISA.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1989年第4期293-297,共5页
Acta Academiae Medicinae Sinicae
