摘要
本文用明胶和肝素亲和层析柱等方法,从猪血浆中分离得到大量(0.5mg/ml)的纤维粘连蛋白(FN)。此FN在琼脂糖电泳和聚丙烯酰胺凝胶电泳中呈单一条带,分子量约450000;巯基乙醇还原后呈现分子量约230000的两条带;可以与抗人和抗牛血浆FN抗体发生免疫沉淀反应;并具有强烈促CHO细胞贴壁活性。提示猪血浆是一种分离提取FN的良好资源。
Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chroma-tography with gelatin-sepharose 4B and heparin-sepharose 4B. The isolated FN shows one single band (MW 450000) both in agarose gel and PAGE, and two similar bands (MW 230000) in the presence of 1% β-mercaptoeth-anol in SDS-PAGE, The FN isolated remains its immunological and biolo- gical properties, being react able with antibodies against human and bovine FN with strong promotion effect on CHO cell adhesion in serum free medium. These data indicate that porcine plasma is a good resource for isolation of FN.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1989年第5期376-380,共5页
Acta Academiae Medicinae Sinicae
