摘要
用高效液相色谱法检测乌头碱的含量具有简便、迅速、灵敏度高、重现性好等特点。应用甲醇-0.01mol/L碳酸铵水溶液-二氯甲烷(90:10:2)为流动相,在ODS反相色谱柱上,以丁卡因为内标,检测波长为235nm,测定家兔血液中的乌头碱,平均回收率96.75±SD5.31％,变异系数5.49％。
Aconitine in rabbit blood was determined by means of UV
detector at 235 nm, using a reversed phase chromatographic column, with me-
thanol-005 mol/L ammonium carbonate-dichloromethane (90:10:2) as mobile
phase, tetracaine as internal standard. The mean recovery was 96.75±SD 5.31%,
and coefficient of variation was 5.49%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1989年第5期211-213,共3页
Chinese Journal of Pharmaceuticals
关键词
乌头碱
高效液相色谱
血药浓度
兔
aconitine, tetracaine, HPLC, blood-drug level, determination
