摘要
用聚丙烯胺凝胶圆盘电泳法对蚯蚓酯酸及过氧化物酶同工酶进行比较研究，结果表明，重金属在蚯蚓体内富集量随污染程度增加而上升，重污染区为中污染区的２．２７倍，为轻污染区的７．３０倍．重污染区Ｃｄ、Ｐｈ、Ａｓ在蚯蚓体内分别达到４７．３、３５．２、１６．３２μｇ·ｋｇ（－１）．其秩相关系数（ｒｓ）＞０．６６－０．７７．蚯蚓酯酶同工酶酶带减少，酶活性降低，而其过氧化物酶酶带和酶活性有所增加．
Esterase and peroxidase isozymes of earthworms in polluted soils are studied with polyacrylamide gel disc electrophoresis. The results show that the heavy metal content in earthworms is increased with increasing pollution level, which is 2. 27 and 7. 30 times higher in heavy polluted soil than in medium and light polluted ones. The Cd, Ph and As contents of earthworms in heavy polluted soil are respectively 47. 3, 35. 2 and 16. 32 μg·kg(-1), and their order coefficient is more than 0. 66-0. 77. The bands and the activity of esterase isozyme are decreased, and those of peroxidase isozyme are increased.
出处
《应用生态学报》
CAS
CSCD
1995年第3期317-322,共6页
Chinese Journal of Applied Ecology
基金
国家自然科学基金
关键词
重金属污染
土壤污染
蚯蚓
同功酶
Heavy metal pollution, Pheretima californica,Esterase isozyme,Peroxidase isozyme,Enzyme activity.
