摘要
本文首次报道利用聚丙烯酰胺凝胶电泳技术分析我国小麦条锈菌11个主要流行生理小种及美国小麦条锈菌白化菌系夏孢子提取物中酯酶、过氧化物酶、过氧化氢酶、碱性磷酸酶、酸性磷酸酶、谷草酰胺转氨酶、多酚氧化酶的同工酶图谱。结果表明:我国小麦条锈菌不同生理小种具有同工酶表型异质性,而同一生理小种的不同单孢系间或不同地理来源的菌系间同工酶表型相同,即生理小种群体内部可能具有同工酶表型同质性。依据同工酶资料的统计分析结果,将所测的中国小麦条锈菌的11个生理小种划为5个类群:A群(条中8号)、B群(条中10号)、C群(条中17号)、D群(条中19、22、23、25、27、26号)、E群(条中28、29号),它反映出小种间遗传基础的相似性及演化特点。结合对美国条锈菌菌系同工酶测定,并与国外同类研究结果比较,揭示出中国小麦条锈菌具有独特的同工酶表型。同工酶表型分析是研究锈菌毒性变异的一个有力工具,建议建立起中国小麦条锈菌毒性菌系多种同工酶标准图谱,用作研究小麦条锈菌毒性变异和群体遗传学的参照系。
Isozyme patterns of 11 Chinese races and an albino strain from America wheat stripe rust (Puccinia striiformis tritici) were analysed and compared by polyacrylamide gel elec-trophoresis. Significant differences of electropheretic pattern of Esterase, Peroxidase, Catalase, Alikaline phosphatase, Acid phosphatase, Glutamate oxalate transaminase and Polyphenoloxidase were detected among 11 Chinese races. Based on the data of isozyme phenotype, the tested races can be divided into 5 groups, namely, A(CY8), B(CY10), C (CY17), D(CY19, 22, 23, 25, 27, 26), E(CY28, 29), which illustrated the evolution of Chinese races of wheat stripe rust. Isolates of the same race from different regions or single spore lines from the same race showed highly isozyme phenotype homogeneity. Chinese stripe rust possessed specific isozyme patterns compared with isozyme patterns of albino strain from America strain in this study and of data abroad. The results indicated that the isozyme patterns of wheat stripe rust were useful geneur markers which were indepen dent of virulence.
出处
《植物病理学报》
CAS
CSCD
北大核心
1995年第2期101-105,共5页
Acta Phytopathologica Sinica
基金
高等学校博士学科点专项科研基金
关键词
小麦
条锈菌
生理小种
同工酶
Wheat stripe rust Physiological race Isozyme pattern
