摘要
从6省13市约18品种草莓样本、分离纯化并研究鉴定,初步证实辽宁、哈尔滨及南京一些栽培田,有草莓伪轻型黄边病毒(SPMYEV)的发生分布。在春香、宝交早生、丹东鸡心,诺宾卡及80-3.1等品种上发病。病样先以小叶嫁接技术,接于草莓UC-4、EMC及“Alpine”,约3～5周显症、下部叶片呈现黄色斑块,接UC-5、EMB不太敏感仅于老叶上现轻斑驳或褪绿斑,一种草莓中瘤钉毛蚜(Chaetosiphon sp.)及棉蚜(Aphis gossypii)作半持久性传播本病毒,桃蚜(Myzus persicae)不传播。UC-4对本病毒的繁殖、保存和嫁接鉴别最为适合,提纯病毒电镜观察其粒子线状,大小约630～680×12～13nm,县典型核蛋白吸收光谱,其最大吸收值位于2.63nm处;最小吸收值位于243nm处。A260/280=1.24。经SDS-聚丙烯酰胺凝胶电泳,测其外壳蛋白分子量约为37,000d。病叶制做超薄切片也观察到线形晶状病毒粒子,分散或聚集于寄主的薄壁组织细胞质中。本病毒系国内首报。
A new strawberry virus has been isolated in China from Chun Xiang(Haru Koari) and Bao-jiao early maturity (Hoko-Wase). After the identification known it was strawberry pseudo mild yellow edge virus (SPMYEV).
The indicative plants were used for small leaf grafting method, those plants were: Fragaria vesca
clone-EMC, UC-4 and 'Alpine' ; and Fragaria virginiana clone-UC-10. The UC-4 and EMC shown
typical yellow blotches and mottles on the leaves of the indicative plant within 20 - 35 days after the grafting has done, it is the symptom induced by SPMYEV.
A kind of strawberry aphid (Chaetosiphon sp. ) which was discovered and collected by us from the strawberry field in Liaoning Province, and cotton aphid (Aphis gossypii) were used for insects transmitting experiment, they were readily transmitted SPMYEV to strawberry plants. Through PEG precipitation, differential centrifugations with sucrose cushion and sucrose density gradient centrifugation process, the virus was successfully extracted and purified. It is filamentous particles; 630-680X12- 13nm. A260/ 280=1. 24. The virus has a single coat protein with M. W. of 37,000 daltons,when detected through the SDS-polyacrylamid gel electrophoresis test. There has also some filamentous crystalized virus particles shown in cytoplasma cells of strawberry by ultrathin sections.
In compliance with whose experiments, these isolates certainly belongs to the SPMYEV. This is the first report in China.
出处
《植物病理学报》
CAS
CSCD
北大核心
1995年第4期367-372,共6页
Acta Phytopathologica Sinica
基金
国家自然科学基金
关键词
草莓
伪轻型黄边病毒
分离
鉴定
蚜虫
Strawberry Pseudo Mild Yellow Edge Virus (SPMYEV) Isolation and Identification Purification Electronmicroscopy and Ultra-thin section Transmitting Aphis
