摘要
本文观察了小鼠肺腺癌ＬＡ７９５母系及不同转移潜能克隆株ＬＡ１、ＬＡ５细胞其胞内［Ｃａ￣（２＋）］及诱导分化剂维胺酸和二甲基亚砜对其影响。发现母系与克隆株细胞间，其胞内静息［Ｃａ￣（２＋）］确有差异（Ｐ＜０．０１）。中转移ＬＡ５克隆株胞内［Ｃａ￣（２＋）］最高，不转移ＬＡ１克隆株最低，母系ＬＡ７０５胞内［Ｃａ￣（２＋）］居中。在克隆株细胞中，胞内［Ｃａ￣（２＋）］与其转移潜能成正比。维胺酸作用不同时间，ＬＡ７９５、ＬＡ１、ＬＡ５胞内［Ｃａ￣（２＋）］先下降，然后逐渐恢复用药前水平，最终至胞内［Ｃａ￣（２＋）］升高。二甲基亚砜作用后，引起胞内［Ｃａ￣（２＋）］一过性升高，然后下降最后又升高。二者均终至所用细胞内［Ｃａ￣（２＋）］升高，提示其诱导分化作用过程中，［Ｃａ￣（２＋）］变化起作用，胞内［Ｃａ￣（２＋）］与肿瘤转移性有一定关系。
The intracellular [Ca￣(2+)] of mouse pulmonary adenocarcinoma LA795cell line and its clone LA1,LA5 were measured using Fura-/AM as fluorescent indica-tor.The results indicated that [Ca￣(2+)]_i was varied.For the clones(nonmetastaticLA1,medium metastatic LA5) [Ca￣(2+)]_i was positively correlated with their metastaticpotential.RⅡ(4-hydroxycarbonphenyl retinoamide)is an differentiation inducer.10￣(-5)MR Ⅱ temporarily decreased [Ca￣(2+)]_i (6h)of all tested cells,then (3d) [Ca￣(2+)]_i re-bound,and finally (7d)[Ca￣(2+)]_i increased,DMSO (dimethyl sulfoxide)induced[Ca￣(2+)]_i to ascend (6h)in all cells,then(3d)[Ca￣(2+)]_i descended, andfinally(7d)[Ca￣(2+)]_i decreased for LA795 cell,increased for LA1 and LA5 cells.The results suggested that [Ca￣(2+)] play a role in the function of retinoamide.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1995年第3期259-262,共4页
Chinese Journal of Pathophysiology
