摘要
本文报道球形芽孢杆菌Ｃ３－４１菌株毒蛋白的生物合成的检测结果。对Ｃ３－４１菌株不同发育阶段发酵液的毒蛋白量进行了酶联免疫吸附测定（ＥＬＩＳＡ），并用生物测定方法检测了发酵液杀蚊毒性，证明其毒蛋白合成量与该菌芽孢的发育和形成密切相关，同时揭示了ＥＬＩＳＡ测出的毒蛋白量与生物测定所示的杀蚊毒力具有很高的符合率。
The toxic proteins in the cultures of Bacillus sphaericus C3-41 were detected by Enzyme-Linked Immunosorbent Assay(ELISA)and the larvicidal potencies of the liguid cultures were also calculated by bioassay.The results showed that synthesis of toxic proteins of Bacillus sphaericus C3-41 was related to the formation of spores.It was found out that toxic protein quantity detected by ELISA conformed to larvicidal potency calculated by bioassay.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1995年第5期337-339,共3页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金
关键词
球形芽孢杆菌
C3-41菌株
杀蚊毒蛋白
合成
Bacillus sphaericus strain C_(3-41) Mosquito-Larvicidal protein Enzyme-Linked Immunosorbent Assay
