摘要
应用￣３Ｈ－ＴｄＲ掺入法、ＥＬＩＳＡ和ＦＡＣＳ研究了ｒＨＩＬ－２调节离体奶牛外周血单核细胞（ｂｏＰＢＭＣ）免疫应答的特点。ｒｈＩＬ－２能诱导细胞持续性增殖和分泌Ｉｇ，尤其是分泌ＩｇＡ和ＩｇＧ＿２（与ＰＷＭ相比，Ｐ＜０．０１）；还能增强ＰＷＭ诱导的Ｉｇ分泌，但不改变ＳＥＢ抑制Ｉｇ分泌的作用。细胞表型也表明，ｒｈＩＬ－２对ＰＷＭ或ＳＥＢ诱导的ｂｏＰＢＭＣ中ＣＤ４￣＋或ＣＤ８￣＋细胞增殖作用无选择性。
ith H-TdR uptake,
ELISA and FACS, the regulative effect of rhIL-2 on the immune
re-sponse of bovine peripheral blood mononuclear cells (boPBMC) in
vitro was studied. rhIL-2alone could stimulate boPBMC continuously to
proliferate and create lg up to d10 ,especially sig-nifican tly
induce boPBMC to secrete lgA and lgG_2 compared with PWM ( P<0. 01 ).
Howev-er, it could also enhance lg production induced by PWM(P <0. 05
), but not change the inhibi-tion of lg production induced by SEB.
The results of the phenotyping showed a increating or adecreating
rate of CD4￣+/CD8￣+on boPBMC induced by PWM or SEB alone or by
either cocul-tured with rhIL-2.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第3期189-191,共3页
Chinese Journal of Immunology
