摘要
人白血病细胞ＨＬ－６０在体外经ＮＩＨ３Ｔ３－ＩＦＮ－α￣＋细胞培养上清作用后或与该细胞共育后其生长受到显著抑制，细胞形态出现空泡，核固缩等现象。给裸鼠皮下接种ＨＬ６０细胞（１×１０￣７／只），当皮下肿瘤结节长至５ｍｍ时，给荷瘤小鼠腹腔内埋植１×１０￣７ＮＩＨ３Ｔ３－ＩＦＮ－α￣＋细胞或腹腔注射阿霉素，结果发现，其皮下肿瘤结节的生长受到非常显著的抑制，特别是当ＮＩＨ３Ｔ３－ＩＦＮ－α￣＋细胞与阿霉素联合应用时，肿瘤生长抑制作用最明显。表明成纤维细胞介导的人ＩＦＮ－α５基因疗法对人白血病有一定的治疗效果，当与适当化疗药物联合应用时疗效更佳。
ecause hIFN-αis
very effective in treating patients with leukemia,we investigated the
effect of fibroblast-mediated hlFN-αgene therapy on the human
leukemia-bearing nude mice. Thegrowth of human HL-60 leukemia cells
were inhibited significantly after HL-60 cells were incu-bated with
the NIH3T3-IFN-α￣+ cells or their ctilture stipernatants.The HL-60
cells were inocu-lated sc into nLide mice, and then the NIH3T3- IFN-
α￣+cells were implaiited ip iiito the leukemi-a-bearing mice or Dox
was also injected ip into the leukemia-bearing mice, The results
demonstrated that the in vivo growth of the leukemia cells was
inhibited obviously,and the better ther-apeutic effect could be
achived when NIH3T3-IFN-α￣+cells were used in combination with
Dox。suggesting that the fibroblast mediated human IFN-α gene
therapy could be used to treat humanleukemia effectively and the
therapeutic effect would be better when it is used with
chemothera-py。
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第4期239-243,共5页
Chinese Journal of Immunology
基金
国家自然科学基金
军队八五攻关基金
关键词
干扰素
基因治疗
成纤维细胞
白血病
阿霉素
Human interferon-αGene therapy Fibroblast
cells Leukemia Adriamycin Chemoimmunotherapy
