摘要
１９９３年夏季，大连水产养殖公司槽式养殖的鲍（Ｈａｌｉｏｔｉｓ）发生脓疱病，死亡率高达５０—６０％。经分离纯化得９３０１和９３０２二个菌株。镜检结果表明，两个菌株均为极生单鞭毛的革兰氏阴性短杆形细菌。在人工感染试验中，健康鲍足肌分别注射两个菌株的菌液，感染率均高达１００％。病症与自然发病的完全相同。因此，证明９３０１和９３０２菌株即为脓疱病的病原菌。药敏试验表明，两个菌株对氯霉素、复方新诺明高度敏感，对氟哌酸中度敏感。它们的ＭＩＣ值分别为１．５６微克／毫升、３．１２微克／毫升、６．２５微克／毫升。联合药敏试验表明，氯霉素与复方新诺明或氟哌酸、复方新诺明与氟哌酸联合后的ＦＩＣ指数均大于０．５而小于１。因此，它们之间的作用均属累加作用。
In summer of 1993,a serious pustule disease occurred in H。discus raised in theartificial sea water ponds at Dalian Marine Culture Commpany.The mortality of the diseasewas as high as 50 60%。Two strains,9301 and 9302,were obtained by isolation and purifica-tion。 With a microscopic examination,it was showed that two strains were all Gram negative,short rod bacteria with a single polar flagellum。suspension of two strains were injected muscu-larly into the foot muscle of healthy H. discus respectively and subjected to infection;the inci-dence of infection was 100%。The signs of disease by artificial infection were quite identical tothat of the disease occurring naturally.Therefore,it indicated that the strains of 9301 and 9302were the pathogens of pustule disease。The drug sensitivity test showed that the two strainswere highly sensitive to chloramphenicol and cotrimoxazole with the values of MIC being l.56ug/ml and 3.12ug/ml respectively,and were moderately sensitive to norfloxacin with thevalue of MIC being 6.25ug/ml.The combined drug sensitivity test showed that when chloram-phenicol and cotrimoxazole or norfloxacin,cotrimoxazole and norfloxacin were used in combina-tion,the values of FIC were all higher than 0.5 and lower than 1.0;So the action betweenthem was an additive effect。
出处
《中国水产科学》
CAS
CSCD
1995年第2期78-84,共7页
Journal of Fishery Sciences of China
关键词
皱纹盘鲍
脓疱病
药敏试验
Haliotis discus,Pustule disease,Drug sensivity test
