摘要
羊开口经提取分离为Ｙ－１、Ｙ－Ｐ、Ｙ－Ａ、Ｙ－Ｅ、Ｙ－Ａ－１和Ｙ－Ａ－１和Ｙ３～１１等部分，通过其对艾滋病毒逆转录酶（ＨＩＶ－ＲＴ）和ＤＮＡ多聚酶（ＤＮＡＰｏｌ）活性试验，发现Ｙ－１、Ｙ－Ａ、Ｙ－Ａ－１、Ｙ－９和Ｙ－１１有极强的抑制酶活性作用。５０％酶活性抑制浓度（ＩＣ５０）对ＨＩＶ－ＲＴ分别为０．１～０．５μｇ／ｍｌ，对ＤＮＡｐｏｌα为０．５～２．５μｇ／ｍｌ。ＨＩＶ－ＲＴ的抑制率当浓度Ｙ－Ａ、Ｙ－Ａ－１２．５μｇ／ｍｌ，Ｙ－９、Ｙ－１１２μｇ／ｍｌ和Ｙ－１５μｇ／ｍｌ时分别为９９．３７％，９３．３５％，９８．２４％，９９．９２％和９０．０％：ＤＮＡｐｏｌα的抑制率当Ｙ－Ａ、Ｙ－９、Ｙ－１１的浓度为２．５μｇ／ｍｌ和Ｙ－１浓度为５μｇ／ｍｌ时，分别为９５．４５％，９３．３３％，９３．０１％和９９．１１％。结果提示羊开口中存在抑制ＨＩＶ－ＲＴ和ＤＮＡｐｏｌα的物质。
The extracts of Y-1、Y-P、Y-A、Y-E、Y-A-1 and Y-3-11 from Melastoma normale were tested for ability to inhibit the activies of HIV reverse transcriptase and human deoxyribonucleic acid(DNA) polymerase.Among 15 fractions,Y-1、Y-A、Y-A-1、Y-9 and Y-11 showed very strong inhibition enzyme activities.The 50% inhibition concentrations(IC50) 0.1～0.5μg/ml for HIV reversetranscriptase and 0.5～2.5μg/ml for DNA polymerase α.HIV reverse transcriptase was inhibited by 99.37%,93.35%,98.24%,99.92%and 89.68% in the presence of Y-A、Y-A-1(2.5μg/ml),Y-9、Y-11(2 μg/ml)and Y-1(5 μg/ml) respectively.DNA polymerase αwas inhibited by 95.45%,93. 33%, 93.01% and 99.11% in the presence of Y-A、Y-9、Y-11(2.5 μg/ml)and Y-1(5μg/ml)respectively.These results indicated that the M.normale contained as vet unidentified substance which inhibited the activities of HIV reverse transcriptase and cellular DNA polymerases.
出处
《中国药科大学学报》
CSCD
北大核心
1995年第2期103-106,共4页
Journal of China Pharmaceutical University
基金
国家自然科学基金
