摘要
采用薄层扫描法测定小蔓长春花组织培养物中长春胺的含量。应用高效硅胶Ｇ薄层板，展开剂为石油醚－氯仿－甲醇（１０：１０：１.２）。在λｓ＝４９０ｎｍ，λＲ＝５５０ｎｍ波长处扫描。长春胺标准品的线性范围为０.５３５～３.７４５μｇ；平均回收率为９８.６８％，ＲＳＤ＝１.９％（ｎ＝６）。小蔓长春花组织培养物经碱性氯仿和２％硫酸提取后点样，按随行外标两点法进行测定，计算样品的含量。测得在固体和液体培养基Ｂ５ａ上生长的由根、茎、叶诱导产生的培养物中长春胺含量分别为：０.６２±０.２２‰、０.３１±０.０７‰、０.３９±０.１０‰和０.５９±０.０９‰、０.５６±０.１５‰、０.５２±０.０７‰。
A method of quantitative analysis of vincamine in tissue culture from Vinca minor L.by dual-wavelength thin-layer scanning methed is reported.Plate of high power sillca gel G was used. Petroleum ether-chloroform-methanol(10:10:1.2) was used as mobile phase.Ameliorative Dragendoff's reagent was used as a detection reagent. Plates adding standard or sample were scanned at λs=490 nm,λR=550 nm in ZIG-ZAG scan mode and vincamine on plate was directly calculated. Lineal correlation of standard cure was rectilineal within the range from 0.535～3.745ug, and average recovery of vincamine was 98.68%, RSD=1.9%( n=6). Vincamine was determined by external standard method(two point). The sample determined was extracted from tissue culture by alkaline chloroform and 2% sulphuric acid.By experiments described above it is demonstrated that vincamine in tissue culture of root, stem, leaf from solid and liquid B5a medium were 0.62±0.22‰,0. 31±0. 07‰,0.39±0. 10‰and 0. 59±0. 09‰,0. 56±0. 15‰and 0. 52±0. 07‰, respec tively.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1995年第6期350-351,共2页
Journal of China Pharmaceutical University
基金
国家医药管理局资助
