摘要
目的:建立能为临床所用的检测游离地高辛的方法,并研究不同肾功能慢性心功能不全患者血清中游离和总地高辛浓度的关系.方法:用超滤及荧光极化免疫测定法检测游离地高辛(Dig).结果:Dig血清标准液浓度为0.96,1.92,3.84 nmol·L^(-1).日内、日间相对标准差分别小于7％和6％,平均回收率为99.95±2.18％.接受Dig治疗的八位慢性心功能不全病人,肾功能不全与正常者的血清Dig游离/总浓度比率分别为63.5±4.7％、75.1±3.9％(P<0.01).结论:肾功能不全病人血清Dig总浓度测定值偏高,提示其血清中存在过量的内源性Dig样免疫活性物质.
AIM:To establish a method for the determination of free digoxin in serum for clinical use and to study the relationship between the free and total digoxin concentrations in chronic cardiac insufficiency patients receiving digoxin with different renal function. METHODS: The ultrafiltration with fluorescence polarization immunoassay was used to determine the concentration of free digoxin. RESULTS: The concentrations of digoxin standards in serum were 0. 96, 1. 92. and 3.84 nmol·L-1. The relative standard deviation was <7 % for intra-day and <6% for inter-day determinations. The average recovery was 99. 95 ± 2.18%. The ratio of free/total digoxin in chronic cardiac insufficiency patients with renal dysfunction was lower than that in patients with normal kidneys (63.5+4.7% vs 75.1+3.9%,P<0.01).CONCLUSION: The present method is simple and reliable. In these patients there is an over-measurement for total digoxin concentration, suggesting the presence of elevated endogenous digoxin-like immunoreactive substances.
出处
《中国药理学报》
CSCD
1995年第1期47-50,共4页
Acta Pharmacologica Sinica
关键词
地高辛
超滤法
肌酸酐
血尿素氮
肾功能衰竭
digoxin
ultrafiltration
fluorescence polarization immunoassay
creatinine
blood urea nitrogen
congestive heart failure
kidney failure
