摘要
采用毛细管电泳-柱端喷壁式安培检测技术,建立了痕量手性药物索他洛尔的分离检测新方法。以1.5%(w/V)羧甲基-β-环糊精为手性选择试剂,借助于环糊精-客体包合物的拆分原理,索他洛尔对映异构体在优化的分离条件:50mmol/LTris-H3PO4缓冲液(pH5.5),分离电压21kV,进样条件18kV/10s,工作电位1150mV(vs.Ag/AgCl),可实现基线分离,线性范围为5～500μg/L;异构体Ⅰ和Ⅱ的检出限(S/N=3)分别为2.0和1.9μg/L。本方法用于模拟血清样品分析,结果令人满意。
A sensitive method based on capillary electrophoresis(CE) with end-column amperometric detection(AD) has been developed for the chiral separation and determination of sotalol enantiomers.Factors affecting the chiral CE separation of the analytes,such as working potential,acidity,concentration of running buffer and the chiral selector,injection and applied voltage,were extensively investigated.The working electrode operated in the wall-jet configuration was a carbon disk electrode(vs.Ag/AgCl).Under the optimum conditions,baseline separation of the enantiomers could be accomplished,and excellent linearity was observed between peak current and concentration of enantiomers in the range from 5 μg/L to 500 μg/L.The limits of detection(S/N = 3) were 2.0 and 1.9 μg/L,respectively.The proposed method has been applied to the analysis of sotalol in human serum samples with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第12期1776-1780,共5页
Chinese Journal of Analytical Chemistry
基金
教育部新世纪优秀人才支持计划(No.NECT-06-0572)
福建省高等学校新世纪人才项目(No.HX-2006-99)资助
关键词
毛细管电泳
安培检测
手性拆分
羧甲基-β-环糊精
索他洛尔
Capillary electrophoresis
Amperometric detection
Chiral separation
Carboxymethyl-β-cyclodextrin
Sotalol
