摘要
为建立超声波提取-高效液相色谱法测定滁菊中木犀草素的方法。采用purospher star RP-18(250 mm×4.6 mm,5μm)色谱柱;以0.1%磷酸溶液(pH=2.6)-甲醇(40:60)为流动相;流速为1 mL/min;检测波长为327 nm。结果表明,木犀草素在10 min内实现快速基线分离,且在0.002~0.05 mg/mL范围内线性关系良好,r=0.9999,平均回收率为99.22%,RSD为1.7%(n=3)。该方法简便快速,可作为滁菊原药材的质量标准。
The method of extraction by ultrasonic and determination by high performance liquid chromatographic of luteolin from Chrysanthemum morifolium Ramat was established.The chromatographic analysis was carried out on a purospher star RP-18 column(250 mm×4.6 mm,5 μm).The mobile phase was phosphate buffer solution(pH2.6) and methanol with isocratic elution(4060).The flow rate was maintained at 1 mL/min.The detection wavelength was set at 327 nm.The results showed that Luteolin was separated quickly within 10 min....
出处
《安徽科技学院学报》
2010年第6期48-51,共4页
Journal of Anhui Science and Technology University
基金
安徽科技学院引进人才基金项目(ZRC2009239)
关键词
滁菊
木犀草素
超声
高效液相色谱法
Chrysanthemum morifolium Ramat
Luteolin
Ultrasonic
HPLC
