摘要
目的从植物浸膏中筛选对乙酰胆碱酯酶有抑制作用的物质,为研发治疗阿尔茨海默病(AD)的新药提供先导化合物。方法建立乙酰胆碱酯酶抑制剂(AchEI)体外筛选模型,用改良Ellman比色法测定AchE的活力,随机筛选多种植物浸膏,对有抑制作用的浸膏进一步进行活性追踪,筛出活性物质。结果追踪得抑制率超50%的两个单体化合物(东茛菪内酯和cannabichromeorcinic acid)。结论东茛菪内酯和cannabichromeorcinic acid可作为治疗AD的先导化合物进行深入研究。
OBJECTIVE To screen out AchE inhibitors from herb extracts. The inhibitors will be used as lead compounds to develop new drugs for Alzheimer’s disease( AD) . METHODS An AchEIs screening model was established in vitro. The extracts of herbs were randomly screened with the improved Ellman colorimetry. The inhibitors were traced from active extracts. RESULTS Two inhibitors,inhibitions of which were more than 50% ,were obtained. They were scopoletin and cannabichromeorcinic acid. CONCLUSION The model is conveni...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第5期542-543,共2页
West China Journal of Pharmaceutical Sciences
基金
新世纪优秀人才基金(NECT-08-0820)
中央高校基本科研业务费专项资金(SWJTU09ZT28)
