摘要
目的采用HPLC法测定左乙拉西坦中的右旋体。方法采用ChiralcelOD-H色谱柱(250mm×4.6mm,5μm);流动相为正己烷-异丙醇-三氟乙酸(90∶10∶0.1);流速1.0mL·min-1;检测波长218nm。结果左乙拉西坦与其右旋体得到了基线分离,其右旋体0.4～3.6×103μg·mL-1与峰面积的线性关系良好(r=0.9998),最低检测限为4ng,定量限为8ng。结论所建方法准确、简便、灵敏,适用于左乙拉西坦的质量控制。
OBJECTIVE To develop an HPLC method for determination of dextro isomer in Levetiracetam. METHODS A Chiralcel OD-H column ( 250 mm × 4. 6 mm,5 μm) was used with the mobile phase of N-hexane-isopropanol-trifluorocetic acid ( 90∶10∶0. 1) at a flow rate of 1. 0 mL·min-1 and the detective wavelength was at 218 nm. RESULTS The separation of both enantiomers of Levetiracetam could be achieved baseline resolution. The calibration curve for Levetiracetam dextro isomer exhibited an excellent linear relationship with ...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第5期593-594,共2页
West China Journal of Pharmaceutical Sciences
