摘要
目的:比较分析几项免疫检测指标在老年Ⅱ型糖尿病患者的变化情况,寻找可以真实反映老年Ⅱ型糖尿病患者机体免疫状况相对理想的指标。方法:运用细胞培养、细胞化学银染技术、全自动化学发光生物芯片分析仪、LA-2000型免疫图象分析系统及流式细胞仪等技术方法,分别检测健康人和老年Ⅱ型糖尿病患者外周血T淋巴细胞核仁形成区银染蛋白(argyrophilic nuclear organizer region associated proteins,Ag-NORs)、T细胞亚群及血浆细胞因子等项目。结果:与健康人比较,老年Ⅱ型糖尿病患者外周血T细胞Ag-NORs降低,CD4、CD4/CD8显著减少,血浆细胞因子IL-6和IL-8降低。结论:经过筛选,T细胞亚群、血浆细胞因子及外周血T淋巴细胞Ag-NORs等单项指标均可在一定程度反映老年Ⅱ型糖尿病患者的免疫状况。
Objective: To identify the changes in cellular immunity in elderly patients with type Ⅱ diabetes, and to seek the ideal indicators which can really reflect immune function of elderly type Ⅱdiabetic patients. Methods: Cell culture, chemieal silver staining of cells, fatal automatic biochip analyzer, LA-2000 immune image analysis system and flow cytometry were used in the monitoring. process Ag-NORs (argyrophilic nuclear organizer region associated proteins) of peripheral T lymphocytes, T cell subsets, plasma cytokines were determined in both healthy volunteers and elderly diabetic patients. Results: Ag-NORs of peripheral T lymphocytes, CD4, CD4/CD8, plasma cytokines in cluding IL-6 and IL-8 were decreased markedly.in diabefic patients compared to normal controls Conclusions:As immune markers, plasma cytokines and Ag-NORs of peripheral T lymphocytes,and T cell subsets might be used individually in clinic but the. the combination of Ag-NORs in T cell , the levels of plasma cytokins and T cell subsets would be more preferable for estimating the immune function of the patients.
出处
《感染．炎症．修复》
2006年第3期135-138,共4页
Infection Inflammation Repair
