摘要
目的:探讨原发性获得性寒冷性荨麻疹(PACU)患者血清组胺释放活性及其意义。方法:通过分离人皮肤肥大细胞和外周血嗜碱粒细胞,进行体外组胺释放试验,测定组胺释放率。用Microcon YM-100超滤膜,将15例PACU患者血清分成分子量大于100kD部分和小于100 kD部分,以及56℃加热去补体等处理部分后,比较不同条件下血清组胺释放活性。结果:15例PACU患者全血清活化肥大细胞或／和嗜碱粒细胞,引起的组胺释放率和阳性率明显高于正常对照组(P＜0．05);大于100kD组与全血清组比较,差异不显著(P＞0．05);小于100kD组和去补体组与全血清组比较,差异显著(P均＜0．05)。PACU患者血清活化肥大细胞或／和嗜碱粒细胞,两组间的组胺释放无显著差异,但是。同一例患者血清活化肥大细胞或／和嗜碱粒细胞,引起组胺释放是不一致的。结论:部分PACU患者血清中存在组胺释放活性,这种活性物质的分子量大于100 kD,且依赖补体的参与。活化肥大细胞和嗜碱粒细胞的介质可能不是同一种物质。
Objective:To evaluate the serum histamine-releasing activity in patients with primary acquired cold urticaria (PACU).Methods:Fifteen PACU patients were studied by using in vitro histamine release assay of mast cells and basophils incubated with the patient's sera.After the removal of the elements larger than and smaller than 100 kD with microcom YM-100 membrane and removal of complement by heating (56℃,30 min).The release of histamine was again determined.Results:Sera from patients with PACU released a significantly higher quantity of histamine compared with the control subjects (P＜0.05).Ultrafiltered fractions (＞100 kD) of sera from patients with PACU retained the histamine-releasing activity,whereas the other fractions (＜100 kD) were unable to induce histamine release.Heating at 56℃for 30 min markedly reduced the histamine-releasing activity of sera from pa- tients with PACU.And histamine release from mast cells was inconsistent with that from basophils in the same patient.Conclusions:Histamine- releasing activity is important in the pathogenesis of PACU by stimulating or fa- cilitating degranulation of mast cells or hasophils.and in some cases complement seems to he essential for hista- mine-releasing activity of serum from patients with PACU.Histamine release from mast cells and basophils might not be mediated by the same factors.
出处
《感染．炎症．修复》
2006年第4期213-217,257,共6页
Infection Inflammation Repair
