摘要
目的探讨C肽对高糖状态下人脐静脉内皮细胞(HUVEC)凋亡的影响,并对凋亡与内皮型一氧化氮合酶(eNOS)表达进行相关性分析。方法内皮细胞凋亡定性用磷脂酰丝氨酸外翻分析(AnnexinV法),凋亡定量采用细胞DNA片段酶联免疫吸附测定法(ELISA)。检测内皮细胞eNOS表达采用半定量逆转录聚合酶链反应(SQRTPCR)法。结果高浓度葡萄糖(33.3mmol/L)使HUVEC凋亡增加(P<0.01),eNOS表达减少,加入生理浓度(1.0nmol/L)或高浓度(50.0nmol/L)C肽后,凋亡显著减少(P<0.05),eNOS表达增加,HUVEC凋亡与eNOS表达相关(r=-0.845,P<0.01)。结论高糖可导致HUVEC凋亡增加;高浓度或生理浓度的C肽可抑制高糖引起的HUVEC凋亡;HUVEC凋亡与eNOS表达呈负相关。
To investigate the effects of C-peptide on apoptosis induced by high glucose and analyze the relationship between the apoptosis and expression of eNOS in HUVEC. Methods We determined the HUVEC apoptosis by Annexin-V, the expression of eNOS mRNA of HUVEC by RT-PCR, the apoptotic quantity of HUVEC by DNA fragment ELISA. Results High level of glucose could increase HUVEC apoptosis （P＜ 0.01） and reduce the expression of eNOS. After adding physiological or high concentration of C-peptide, the apoptosis decreased and the expressions of eNOS increased markedly, with the relationship coefficient of -0. 854 （P＜0.01）. Condusion High glucose may induce apoptosis. Physiological or high concentration of C-peptide may reduce the apoptosis induced by hyperglycemia, the relationship between apoptosis and eNOS is negative.
