摘要
目的:比较老年人、成人和胎儿骨髓间充质干细胞(MSCs)的生物学性状,为选择抗砷细胞的种子细胞提供实验依据。方法:取老年人、成人和胎儿骨髓MSCs,在α-MEM培养液中进行骨髓MSCs培养,测定生长曲线、细胞贴壁率及NaAsO2对骨髓MSCs的细胞毒作用。结果:老年人、成人和胎儿骨髓MSCs在细胞形态、生长特性等方面是相似的,胎儿骨髓MSCs的扩增潜能明显强于成人和老年人骨髓MSCs,对NaAsO2的耐受性也较成人和老年人骨髓MSCs高。结论:从老年人、成人及胎儿骨髓中可分离培养出骨髓MSCs,在体外保持有效扩增能力。胎儿骨髓MSCs较成人和老年人骨髓MSCs更原始,具有更大的体外扩增潜能,可做为抗砷细胞的种子细胞。
To explore the difference of biological characteristics between human bone marrow derived mesenchymal stem cells of human fetal, adult and aged people. Methods： Cells from bone marrow of 6 months fetal, adult and aged people were cultured in α-MEM medium to obtain mesenchymal stem cells. The growth curve, the adherent ability and the effect of NaAsO2 on MSCs were investigated. Results： The adherent fetal, adult and old bone marrow-derived cells raised the population of cells with features of MSCs. These MSCs were similar in cell morphology. The proliferation of the bone marrow derived MSCs from the fetus was higher than those from the adult and the o[d. The arsenic-resistance abi[ity of the MSCs from the fetus was higher than that from the adult and the old. Clonclusion： Bone marrow derived MSCs should be enough to sustain a steady supply of cells for proliferation. Fetal bone marrow derived MSCs can be used as arsenic-resistance cells.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2005年第4期400-402,共3页
Chinese Journal of Anatomy
基金
国家自然科学基金(30360095)
