摘要
本文采用受体放射自显影技术，对氟哌啶加强针刺镇痛时脑内阿片受体结合位点的变化进行观察，受体的标记配基为３Ｈ－依托芬。结果表明，针刺镇痛时大鼠脑内３Ｈ－依托芬的结合位点在许多核团明显增加，如中脑导水管周围灰质、尾核、伏核、视前区、海马、缰核、丘脑、下丘脑、上丘、脚间核、黑质、杏仁核、脊髓背角等，当氟哌啶与针刺合用并加强了针刺镇痛时，上述核团内阿片受体的结合位点进一步增加。本文结果提示，内阿片受体的激活是实现针刺镇痛的条件之一，内阿片受体功能的进一步增强，可能是氟哌啶加强针刺镇痛的机制之一。Ｐ＜０．０５．ｖｓＮＳｇｒｏｕｐ；＋Ｐ＜０．０５，＋＋ｐ＜０．ｏｌ，ｖｓＮＳ＋ＥＡｇｒｏｕｐ；＊Ｐ＜０．０５，＂ｕｐ＜０．ｏｌ．ｖｓＤｇｒｏｕｐ￣ＳｅＯｆＭ￣ｄｅｒｐｔｈｅｂ￣２０ｒａｔＳｗｅｒｅｄｉｖｉｄｅｄｉｎｔｏｆｏｕｒｇｒｏｕｐｓａｓｍｅｎｈｏｎｅｄａｂｏｖｅ．ＴｈｅａｎｉｍａｌｓｗｅｒｅＳａｃｒｉｆｉｃｅｄｂｙｄｍｐｉｔａｈｏｎａｔｔｈｅ￣ｍｕｍｏｆｐａｉｎ￣ｏｌｄａｐｐｅａｌｓ（ａｂｏｕｔ２０ＩｎｉｎｆｏｌｌｏｗｉｎｇＥＡ）．Ｐａｉｎｔｏｅｈｏｌｄｗａｓｍｅａ￣ｕｎｔｉｌｔｈｅｄａｓｐｉｔａｈｏｎ．
By using autoradiographic technique, the binding sites of opioid receptors were observed when acupuncture analgesia (AA) was potentiated by droperidol, an antagonist of dopamine receptors. Labelled ligand for opioid receptors was 3H -etorphine (non-selective ligand of opioid receptors). The results showed that the binding of 3H-etorphine significantly increased after acupuncture in many brain regions, such as periaqueductal gray (PAG), caudate nucleis,accumbens, preoptic area, hippocampus, amygdala, thalamus, hypothalamus, interpeduncular nucleus, superior colliculi, substantia nigra, dorsal horn of the spinal cord, etc. When droperidol was combined with acupuncture and potentiated AA, the binding of 3H -etorphine further increased in the above nuclei. The results in the present study suggest that the activation of opioid receptors is one of the mechanisms of AA, and the further enhancement of the function of the opioid receptors by droperidol may underlie the mechanisms of droperidol potentiation of AA.
出处
《神经科学》
SCIE
CAS
1995年第1期7-13,共7页
Chinese Journal of Neuroscience
关键词
内阿片受体
氟哌啶
针刺镇痛
放射自显影
opioid receptor
acupuncture analgesia
droperidol
autoradiography
