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吗啡预处理对成年大鼠心肌细胞缺血-再灌注损伤的保护作用 被引量:6

Effect of morphine preconditioning on ischemia-reperfusion injury in cultured adult rat cardiac myocytes
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摘要 目的探讨吗啡预处理对培养大鼠心肌细胞模拟缺血-再灌注损伤的保护作用及其与剂量的关系.方法采用培养成年大鼠心肌细胞模拟缺血-再灌注损伤模型,观察不同浓度的吗啡预处理对心肌细胞缺血-再灌注损伤后细胞成活率和肌酸激酶(CK)释放的影响.结果吗啡(0.1~10μmol/L)预处理培养大鼠心肌细胞后,能显著减少模拟缺血-再灌注损伤引起的心肌细胞死亡和CK的释放(P<0.01).在0.1~1 μmol/L的浓度范围内,吗啡预处理对心肌细胞的保护效应呈剂量依赖性增加.结论在0.1~1μmol/L的浓度范围内,吗啡预处理能剂量依赖地产生心肌保护效应.这提示吗啡在整体心脏预处理心肌保护效应可在成熟的心肌细胞水平起作用. Objective To investigate the relationship between dose of morphine and protection of morphine preconditioning from simulated ischemia-reperfusion injury in cultured adult rat cardiac myocytes. Methods In the model of cultured adult rat cardiac myocytes suffered from simulated ischemia-reperfusion injury, the myocytes were pretreated with morphine in concentration from O. O1 μmol/L to 10 μmol/I.,and then simulated ischemia for 40 rain and reperfused for 30 rain. The cell viability and creatine kinase(CK) released from the cells were measured by MTT and CK test kit respectively. Results Morphine pretreatment was able to protect cardiomycytes against simulated ischemia-reperfusion injury by increasing the number of live cells and decreasing the release of CK from the cardiomyocytes. In the concentrations from 0.1 μmol/L to 1 μmol/L, the effects of morphine pretreatment on protecting cardiomyocytes against simulated ischemia-reperfusion injury were increased in a dose-dependent manner. Conclusion Morphine (0.1-1 μmol/L) can dose-dependently protect the cardiomyocytes from simulated ischemia-reperfusion injury. The results provide a direct evidence that the preconditioning-like effect of morphine in the intact heart can be exerted in adult cardiomyocytes.
出处 《临床麻醉学杂志》 CAS CSCD 2005年第8期546-548,共3页 Journal of Clinical Anesthesiology
基金 国家自然科学基金资助课题(No30200089)
关键词 吗啡 预处理 成年 大鼠 心肌细胞 缺血-再灌注损伤 肌酸激酶 保护作用 Morphine Cardiac myocytes Reperfusion injury Ischemic preconditioning
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  • 1D.L.斯佩克特 L.A.莱因万德 R.D.戈德曼著.黄培堂等译.细胞实验指南:上册[M].北京:科学出版社,2001.83-89.
  • 2蔡文琴 王伯V.免疫组织化学和核酸分子杂交技术[M].成都:四川科学技术出版社,1994.61-66.
  • 3许秀芳,李温斌,陈宝田,吕燕宁,赵莉敏,陈燕.成年大鼠心肌细胞培养方法的建立和形态学观察[J].首都医科大学学报,2000,21(2):104-107. 被引量:19

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