摘要
目的:研究刺梨多糖(RoseroxburghiiTrattpolysaccharide,RRTP)对神经干细胞谷氨酸损伤的保护作用。方法:采用离子交换柱层析和凝胶柱层析纯化得到RRTP;取出大鼠胚胎的纹状体进行神经干细胞培养,神经干细胞谷氨酸损伤模型模拟神经元的缺血缺氧性损伤。实验分正常对照组,谷氨酸损伤组和RRTP不同浓度试验组。观察谷氨酸损伤后各组神经干细胞死亡率和乳酸脱氢酶漏出率。结果:不同浓度RRTP的试验组神经干细胞损伤有不同程度的减轻。高浓度RRTP试验组神经干细胞死亡率和乳酸脱氢酶漏出率较低,与谷氨酸损伤组比较有显著性差异(P<0.01)。结论:RRTP对神经干细胞损伤有明显的保护作用。
Objective： To study the protective effect of polysaccharide from Rose roxburghii Tratt （RRTP-1） on neural stem cells damaged by glutamic acid （Glu）. Method： RRTP-1 was obtained by ion exchange column chromatography and gel chromatography. The neural stem cells were cultured from the striata of fetal mice and damaged by glutamic acid to simulate hypoxic-ischemic brain injury. The cultures were divided into control group, glutamic acid group and test groups in which different doses of RRTP-1 were added with glutamic acid. The death rate of neural stem cells damaged by Glu and LDH leakage were observed. Results： The neural stem cells damage of test groups were alleviated, and the death rate of neural stem cells and LDH leakage of high dose test group were significantly lower than glutamic acid group. Conclusion： RRTP-1 has remarkable protective effect on neural stem cells damage.
出处
《营养学报》
CAS
CSCD
北大核心
2005年第4期339-341,共3页
Acta Nutrimenta Sinica
基金
贵州省优秀科技教育人才省长专项资金
关键词
刺梨多糖
分离纯化
神经干细胞
谷氨酸损伤
polysaccharide
Rose roxburghii Tratt
purification
neural stem cells
glutamic acid damage
