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HPLC法同时测定曲咪新乳膏中硝酸咪康唑和醋酸曲安奈德的含量 被引量:10

HPLC Simultaneous Determination of Triamcinolone Acetonide Acetate and Miconazole Nitrate in Triamcinolone Acetonide Acetate,Miconazole Nitrate and Neomycin Sulfate Ointment
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摘要 目的:建立高效液相色谱法同时测定曲咪新乳膏中硝酸咪康唑和醋酸曲安奈德含量的方法。方法:用 ZORBAX SB-C_(18)(4.6mm×250mm,5μm)色谱柱,以甲醇-乙腈-0.5%醋酸铵溶液(43:43:14)为流动相,流速为1.1 mL·min^(-1),柱温为25℃,检测波长为235nm。结果:硝酸咪康唑和醋酸曲安奈德的保留时间分别为8.6 min和3.6 min;理论板数分别为11400和9100;HPLC 法测定的线性范围分别为10~200μg·mL^(-1)(r=0.9999)和1~20μg·mL^(-1)(r=0.9999)。本方法的精密度和稳定性良好,RSD<2%。结论:本法简便、快速、准确、可靠,用于曲咪新乳膏中硝酸咪康唑和醋酸曲安奈德的含量测定。 Objective: To establish an HPLC method for determination of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide acetate, miconazole nitrate and neomycin sulfate ointment at the same time. Methods: The column : ZORBAX SB - C18 (4.6 mm × 250 mm, 5 μm) ; The mobile phase : methanol - acetonitrile - 0. 5 % acetate solution (43: 43: 14 ) ; The flow rate : 1. 1 mL · min^-1 ; The column temperature:25℃; The detection wavelength :235 nm. Results :The retention times for miconazole nitrate and triamcinolone acetonide acetate were 8.6 min and 3.6 min with theoretical plates were 11400 and 9100 ,respectively. There were good linear relationship with in the range of 10 -200 μg · mL^-1 (r =0. 9999) and 1 -20μg · mL^-1 (r =0. 9999). The precision and repeatability of the method was good, RSD 〈 2%. Conclusion : The method is convenient, rapid, accurate and reliable and may be adopted for assay of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide acetate, miconazole nitrate and neomycin sulfate ointment.
作者 孙丽
出处 《药物分析杂志》 CAS CSCD 北大核心 2005年第8期946-948,共3页 Chinese Journal of Pharmaceutical Analysis
关键词 曲咪新乳膏 硝酸咪康唑 醋酸曲安奈德 高效液相色谱法 HPLC法 同时测定 含量测定 检测波长 保留时间 色谱柱 triamcinolone acetonide acetate,miconazole nitrate and neomycin sulfate ointment miconazole nitrate triamcinolone acetonide acetate HPLC
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