摘要
利用花粉管通道技术将携带有菜豆几丁质酶基因的质粒pBch转化大豆品种绥农10号,在转化的D4代出现白化致死突变株系。通过同工酶技术、PCR技术和RAPD分析方法对白化致死突变系进行了研究,结果表明:白化致死突变株各个部位的SOD酶活性、POD酶活性均高于正常株,在根中表现尤为明显;PCR扩增结果表明在白化致死突变株系中存在几丁质酶基因,证明转化成功;RAPD方法初步分析了大豆白化致死突变株与正常植株间的差异,获得750bp差异片段。
Soybean cultivar Suinong 10 had been transformed by pBch plasmid haboring chitinase gene through pollen tube pathway method. A albinos lethal mutant line appeared in D4 generation . Research has been done on albinos lethal mutant line by Isozyme analysis,PCR and RAPD techniques. The results showed as follows：that the enzyme activity of SOD and PPO in albinos lethal mutant line was higher than that in normal plant, and that was much more obvious in roots; chitinase gene had been amplified by PCR in albinos lethal mutant line, that certificated transformation was successful; Reason of soybean albinos lethal had been primarilly analysed using RAPD in molecular level, obvious differences are existed between genomes of normal plant and albinos lethal plant.
出处
《大豆科学》
CAS
CSCD
北大核心
2005年第3期229-231,F0003,共4页
Soybean Science
