摘要
设计了1对特异性引物,应用降落PCR法对减蛋综合症病毒(EDSV)六邻体蛋白基因进行了扩增。将扩增产物克隆至pMD18-T载体中,经酶切和PCR鉴定后,初步证明了目的片段的正确性。进一步经核苷酸序列测定表明,所扩增的基因片段长度为2.74kb,共编码910个氨基酸,与由EDSV弱毒株(AA-2)全基因组的Hind酶切片段测序所得到的六邻体结构基因推测的氨基酸序列相比,有11处氨基酸发生了变异。
Hexon-ecoding protein gene of egg drop syndrome virus （EDSV） was amplified by touchdown PCR（TD-PCR） and was cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The sequence analysis showed that the nucleotide sequence of hexon protein gene was 2.74 kb and encoding a protein of 910 amino acids, 11 amino acids were different from the hexon protein sequenced from the Hind Ⅲ -D fragment of egg drop syndrome virus AA-2 strain.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2005年第8期31-34,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
河南省自然科学基金资助项目(0214040027)
