摘要
背景与目的p120ctn可以通过改变RhoGTP酶的状态进而调节细胞的黏附和肿瘤的转移。本研究通过观察p120ctn与RhoGTP酶中RhoA、Cdc42在非小细胞肺癌中的表达及其对临床病理特征的影响,为探索p120ctn在肿瘤中的生物学功能提供依据。方法采用S-P免疫组化法检测124例非小细胞肺癌标本中p120ctn、RhoA和Cdc42的表达。结果正常支气管粘膜细胞p120ctn为细胞膜强阳性表达,在非小细胞肺癌出现胞膜表达减弱和胞浆表达等异常表达,其异常表达率为79.8%(99/124),异常表达与组织类型无明显关系,与肿瘤细胞的分化、TNM分期和淋巴结转移有明显关系(P<0.05)。RhoA和Cdc42在正常支气管粘膜中均正常表达,在癌组织中表达明显增强(62.9%,78/124;56.5%,70/124)。RhoA、Cdc42在非小细胞肺癌中的表达增强与临床病理特征无明显关系。非小细胞肺癌中p120ctn的异常表达与RhoA和Cdc42的表达增强有较好的一致性和相关性(Kappa=0.523,P<0.001;Kappa=0.493,P<0.001)。结论p120ctn的异常表达可能是导致RhoA和Cdc42表达增强的重要因素。
Background and objective Cell adhesion and tumor metastasis can be regulated by p120^ctn. The aim of this work is to investigate the correlation of expression of p120^ctn, RhoA and Cdc42, as well as their influence on clinical pathologic factors in non-small cell lung cancer, and to provide evidences for exploring the biological functions of p120^ctn in tumors. Methods The expression of p120^ctn, RhoA and Cdc42 was detected in 124 cases of non-small cell lung cancer by immunohistochemistry. Results The p120^ctn mainly expressed on the cell membrane of normal bronchial epithelium. Abnormal expression of p120^ctn , including decreased membranous expression and increased cytoplasmic expression, was found in 99 of 124 lung cancer specimens （79.8% ）. The abnormal expression rate of p120^ctn evidently related to cell differentiation, TNM stages and lymph node metastasis （P〈0.05）, but not to histological classification. The RhoA and Cde42 expressed normally in bronchial epithelium, but apparently overexpressed in tumor tissues （62.9%, 78/124; 56.5%, 70/124）. The overexpression rate of RhoA and Cde42 had no significant relationship with clinical pathological characteristics. There were considerable consistency and correlation between abnormal p120^ctn expression and overexpression of RhoA and Cdc42 in non-small cell lung cancer （Kappa=0. 523, P〈0. 001 ; Kappa=0. 493, P〈0. 001）. Conclusion The abnormal expression of p120^ctn may play an important role in the up-regulation of RhoA and Cdc42 expression.
出处
《中国肺癌杂志》
CAS
2005年第4期304-308,共5页
Chinese Journal of Lung Cancer
基金
国家自然科学基金(No.30470764)资助~~
