摘要
为了确定卵泡液(直径大于10mm)对卵母细胞成熟的影响,共收集1759枚卵母细胞进行试验。在成熟液中分别添加0%,10%,30%和50%的卵泡液(bFF,bovinefollicularfluid),成熟培养24h后,孤雌激活或者体外受精和胚胎培养;对比10%bFF和10%胎牛血清(FBS),以及观察微滴培养对卵母细胞成熟的影响。结果表明,在孤雌激活组中,bFF浓度对卵裂率的影响差异不显著,但胚胎囊胚发育率高于对照组(P<0.05);在体外受精组中随着bFF浓度增加而卵裂率下降(P<0.01),而且囊胚率也下降(P<0.05)。在10%的bFF组中,孤雌激活囊胚率和体外受精囊胚率分别为47.1%和38.0%,囊胚扩张率显著高于其它组。从试验得出,添加10%bFF有利于卵母细胞胞质成熟,且不会降低到受精率,而且可以替代FBS;微滴培养不利于卵母细胞成熟。
The effect of follicular fluid （〉10mm） on bovine oocyte maturation in vitro was evaluated by addition of different concentration （0, 10, 30 and 50%, respectively） of bFF to the maturation medium. A total of 1759 oocytes collected from abattoir ovaries were used. After 24h of maturation, the oocytes were activated by parthenogenesis （PG） or in vitro fertilization （IVF） and cultured ; Comparison of 10% bFF and 10% FBS with oocytes maturation; Efficiency of micro-drop culture was assessed. The results showed cleavage rates did not differ in parthenogenesis group （P〉0.05）, but embryo blastocysts rates was significantly greater than control （P〈0.05）, especially with 10% bFF; A significant decrease in cleavage rates （P〈0.01） and blastocyst rates （P〈0.05） was found with bFF concentration increase in IVF group. The rate of PG blastocyst and IVF blastocyst was the highest with the addition of 10% bFF （47.1% and 38.0%, respectively）, more expanded blastocysts were gotten than others. In conclusion, addition of 10% bFF is benefit to the cytoplasmic maturation and can not hamper fertilization ability; bFF can be used in the IVM medium in place of FBS; Micro-drop culture do not increase the rate of the oocyte maturation.
出处
《中国农学通报》
CSCD
2005年第8期7-9,共3页
Chinese Agricultural Science Bulletin
基金
国家"863"高技术项目(2001AA213081)
关键词
卵泡液
卵母细胞
牛
发育潜力
成熟质量
胚胎
营养物质
Bovine, Embryo, Follicular fluid, Embryo culture, In vitro fertilization, Parthenogenetic activation
