摘要
目的:观察持续性高正加速度导致咀嚼肌损伤的自然恢复。方法:雄性Wistar大鼠108只随机分成9个不同处理条件的实验组。A组为空白对照。B组固定5min,不上机。C组用特制的固定装置固定于“2000”型动物离心机的转臂上,+1Gz离心旋转5min。其余各组+10Gz峰值持续时间为30sec,间隔时+1Gz离心60sec,连续5次/d,4d/wk,共4wk。A、B、C、D组离心处理后次日断头处死,其余各组分别于离心处理后1、2、4、8、12周后次日断头处死,各组动物分别取咬肌和颞肌作光镜和透射电镜观察。结果:A组、B组和C组颞肌和咬肌未见异常。D组颞肌和咬肌肌纤维的电子密度规律性分布较差。肌间线粒体肿胀,基质电子密度降低、出现髓样结构、致密结构或空化。E组颞肌和咬肌肌纤维肌节完整,肌原纤维排列整齐,线粒体嵴多而长,肌纤维膜无异常。F、G、H、I组肌纤维结构未见异常。结论:持续性高+Gz重复作用导致大鼠颞肌和咬肌的损伤能很快得到自然修复。
Objectives： To obscrve the post-injury regenerating ability of masticatory muscles of Wistar rats exposed to repeatcd ＋Gz forces. Methods： A hundred and eight male rats were randomly assigned to 9 groups with 12 rats in each group. Group A was normally fed as the blank control group. Group B was only fixed with rat-kept devices for 5 minutes as the fixed control group. Group C were borne ＋1Gz for 5 minutes. Group D, E, F, G, H and 1 were repeatedly exposed to ＋10Gz 30 minutes each fbr 5 times a day with ＋1Gz for 1 minute as intervals. The rats in Group A, B, C and D were killed on the next day after the centrifugation. The others in Group E, F, G, H and I were killed at 1, 2, 3, 4,8 and 12 weeks after the centrifugation, respectively. Samples of temporalis and masseter muscles were taken for light and transmission electron-microscope observation. Results： No abnormal changes were observed in Group A, B and C. In Group D, muscular fiber presented irregular electron densities. Electron-microscope observation of muscle samples in E group disclosed a basically normal structure. No abnormalities were found in Group F, G, H and I. Conclusion： It is confirmed that repeated ＋Gz exposure could induce injury to rat temporalis and masseter muscles, which could be self-repaired soon.
出处
《口腔颌面修复学杂志》
2005年第3期165-167,共3页
Chinese Journal of Prosthodontics
基金
军队医药卫生科研基金(01Q061)
关键词
加速度
咀嚼肌
颞肌
咬肌
损伤/修复
恢复
acceleration
masticatory muscles
temporal muscle
masseter muscle
injury/repairment
resume
