摘要
目的:探讨转化生长因子β1(TGFβ1)在大鼠周围神经损伤后再生过程中的作用及是否参与了再生中脊髓前角运动神经元碱性成纤维生长因子(bFGF)表达的调节。方法:将48只成年SD大鼠在右侧坐骨神经压榨损伤术后随机分为TGFβ1组和生理盐水(NS)组。TGFβ1组在坐骨神经压榨损伤近心端注射TGFβ150μL(0.1μg/mL),术后隔日在右侧下肢后群肌内注射TGFβ150μL,对照组同样方法注射等量NS。实验动物分别存活3,7,14和21d后运用免疫组织化学方法检测TGFβ1对脊髓前角运动神经元bFGF蛋白的表达变化。存活21d的实验动物还用半薄切片和FastBlue逆行荧光示踪方法观察TGFβ1对坐骨神经损伤后远端的再生情况。结果:TGFβ1组脊髓前角bFGF蛋白的表达高于NS组(P<0.05);损伤远侧端再生轴突数目、直径与髓鞘厚度TGFβ1组均明显好于NS组(P<0.05);TGFβ1实验组脊髓和背根节内荧光标记细胞数量明显多于NS组(P<0.01)。结论:外源性TGFβ1可以促进周围神经损伤后再生;TGFβ1上调周围神经损伤后脊髓前角运动神经元bFGF的表达。
Objective To explore the effects of exogenous transforming growth factor-beta 1 （TGFβ1 ） on peripheral nerve regeneration after the peripheral nerve injury and if TGFβ1 regulates the expression of basic fibroblast growth factor （bFGF） in the anterior horn motoneurons of spinal cord during regeneration. Methods Forty-eight rats were crushed on the right sciatic nerve and then randomly divided into 2 groups：TGFβ1 group and NS group. In TGFβ1 group, TGFβ1 50 μL （0. 1μg/mL） was injected into the proximal nerve near to the crushed nerve and after the operation the injured leg was injected with equal TGFβ1 whereas the NS was replaced in the NS group. The rats of each group survived for 3, 7, 14 and 21 days after the lesion. The bFGF expression in the anterior horn motoneurons of spinal cord was detected by immunohistochemistry （IHC）. Semi-thin section and Fast Blue retrograde tracing were also performed with the rats surviving for 21 days to observe the regeneration of distal end in the injured right sciatic nerve. Results The number of bFGF immunoreactive positive motoneurons in TGFβ1 group was obviously higher than that of the NS group（ P〈0.05）. In the distal sciatic nerve of the rats treated with TGFβ1, the number and diameter of regenerating myelinated axons and the thickness of myelinated sheath were more than those of the NS group （P〈0.05）. The number of motoneurons in spinal cord and neurons in dorsol root ganglia （DRG） labelled with Fast Blue in the NS group was obviously lower than in the TGFβ1 group （P〈0.01）. Conclusion Exogenous TGFβ1 plays an important role in pro-moting the peripheral nerve regeneration;TGFβ1 up-regulates the bFGF expression in the anterior horn motoneurons of spinal cord during the peripheral nerve regeneration.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2005年第4期447-451,共5页
Journal of Central South University ：Medical Science
关键词
转化生长因子Β1
碱性成纤维生长因子
坐骨神经
再生
transforming growth factor-beta 1
basic fibroblast growth factor
sciatic nerve
regeneration
