摘要
对经高静水压处理的水稻粤香占(Oryza sativaL.cv.Yuexiangzhan)种子播种后的植株进行了抗氧化酶活性的测定和对逆境胁迫响应的研究。结果表明,高压处理抑制了水稻生长早期(12 d)色素和蛋白质含量的增加,并使早期抗氧化酶活性表现较对照低。随着播种天数增加,经高压处理的材料中的色素、蛋白质含量不同程度地增加,抗氧化酶活性发生改变,至播种后32 d时,高压处理的叶绿素含量和可溶性蛋白含量都高于对照粤香占。在自然低温条件下,经高压(干压、湿压)处理的植株其Rubisco大、小亚基含量和光合色素含量较高,抗光抑制能力增强,具一定的耐低温性。
Rice seeds （Oryza sativa L. cv. Yuexiangzhan） were soared for 10h or not soaked then exposea to 75MPa high hydrostatic pressure （HHP） for 12h. The seeds treated by HHP and their control were sowed in the field. Activities of antioxidative enzymes （SOD, AsA-POD） in the leaves, Rubisco content, Fv/Fm and Φ PSⅡ were measured to evaluate the effect of HHP on the growth and development of rice. Results indicated that HHP inhibited the increasing of pigment and protein content in the early growth stage（12d）. Activities of antioxidative enzymes were lower than that of the control at the moment. With days after sowing increasing, the content of pigment and protein in the treatment were higher than that of the control （Yuexiangzhan）. Antioxidative ability of rice treated by HHP was stronger than that of the control at the 32th days after sowing. The content of Rubisco,including Rubisco large subunit and Rubisco small subunit, was higher in the treated rice than that of the control at the natural low temperature. Fv/Fm and ΦPSⅡ of treatment and control decreased at noon but can recover at evening, and recovery of treatment was better than that of the control. These results suggested that difference in growth between the treatment and control was due to the effect of HHP on the activities of antioxidative enzymes of rice possibly. Furthermore, HHP may enhance rice＇s resistance to low temperature.
出处
《高压物理学报》
EI
CAS
CSCD
北大核心
2005年第3期235-240,共6页
Chinese Journal of High Pressure Physics
基金
国家自然科学基金(30471002)
中国科学院农业项目办公室重点资助项目
国家重大基础研究发展项目(G1998010100)
关键词
水稻
高静水压
抗氧化
胁迫
rice
high hydrostatic pressure
antioxidative
stress
