摘要
给母代种鸡饲喂添加不同剂量Ｇｅ－１３２（５０、１００、１５０、２００ｍｇ／ｋｇ）的饲料，获取４组富锗种蛋（含Ｇｅ量为（５８．１８９２±４．９２３４）～（７６．０３５０±８．０９６５）μｇ／枚蛋），以其与１组普通种蛋（含Ｇｅ量（１．５９９１±０．２６２４）μｇ／枚蛋）孵出的雏鸡，编为Ⅰ～Ⅴ组，每组１００只；再将各组分为２个小组各５０只，分别编号为Ⅰ－１、Ⅰ－２以至Ⅴ－１、Ⅴ－２小组；其中Ⅰ－１至Ⅴ－１各小组按常规程序进行免疫，而Ⅰ－２至Ｖ－２各小组不进行免疫。于１、７、１４、２１及２８日龄时，各小组随机抽取６只分别采血检测ＳＯＤ、ＧＳＨ－Ｐｘ活力及ＬＰＯ的含量。结果显示：（１）１４日龄前，Ⅰ～Ⅳ组雏鸡血液ＳＯＤ及ＧＳＨ－Ｐｘ活性均明显高于对照（Ⅴ）组，而ＬＰＯ含量则显著低于Ⅴ组，１５日龄后组间差异不显著；（２）Ⅰ－１至Ⅴ－１小组与Ⅰ－２至Ⅴ－２小组比较未见明显规律性差异，表明免疫对以上３项指标无明显影响；（３）相关和回归分析结果表明，种蛋中锗含量与１日龄雏鸡血液ＳＯＤ及ＧＳＨ－Ｐｘ活性呈强正相关（ｒ分别为０．８９７和０．９３９），而与血清ＬＰＯ含量则呈强负相关（ｒ为—０．８７４）。７日龄后，这些相关性即明显减?
Parent laying hens were fed the diets added four different amounts of Ge-132 to obtainthe eggs rich in germanium. The vertical influence of germanium on antioxidant function of filial chick-ens hatchedfrom these eggs were studied.The chickens hatched from rich-gerinanium eggs of fourgroup[(58· 189 2± 4.9234)to(76.0350 ± 8.096 5)μg Ge per egg] and normal eggs[(1.599 1±0.262 4)μg Ge per egg)were randomly divided into the correspanding groups,i.e.groups Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ respectively,100 chickens in each group.Each group was randomly subdivided into twogroups numbered from Ⅰ-1,Ⅰ-2 to Ⅴ-1,Ⅴ-2.The chickens from group I-1 to group Ⅴ-1 were vac-cinated by the normal immunization procedure,and those from group Ⅰ-2 to group Ⅴ-2 were not vacci-natcd. All chickens were managed under the same standard conditions,Blood samples were collected atlst,7th,14th,21st and 28th dav after immunization and serum LPO contents,serum SOD and bloodGSH-Px activities were determined.The experimental results showed as follows:1.The activities ofserum SOD and blood GSH-Px of group Ⅰ～Ⅳ Wwere significantly higher than those of group Ⅴ,butthe contents of serum LPO of group Ⅰ～Ⅳ were evidently lower than that of group Ⅴ in chickens aged14 days and under.2.The changes of serum SOD,LPO and blood GSH-Px activities had no significantdifferences among groups.3.At any time, the regular and significant difference between group Ⅰ-1 ～ Ⅴ-1 and group I-2～Ⅴ-2 were not observed, which indicated that the immunization did not signifi-cantly affect the activities of serum SOD and blood GSH-Px or serum LPO contents.4.There were sig-nificantly positive correlation between the germanium contents of eggs and the activities of serum SODand blood GSH-Px(r=0.899and r=0.939)and negative correlation between the germanium contentsof eggs and serum LPO levels(r=0.894)at the age of one day by regressive and relative analysis,but the correlation significantly declined under the age of seven days. The results confirmed that germa-nium might significantly enhance the antioxidant function of day-old chickens. Moreover,this influenceapproximately lasted from one to two weeks.
出处
《中国兽医学报》
CAS
CSCD
1996年第2期151-155,共5页
Chinese Journal of Veterinary Science
基金
黑龙江省教委课题
关键词
鸡
雏鸡
锗
抗氧化作用
繁殖
孵化
germanium
antioxidant function
SOD
GSH-Px
LPO
chicken
