摘要
采集５６头丹麦长白猪（其中１头为１周龄的仔猪、２６头种公猪、２９头种母猪）猪毛样品，粗提ＤＮＡ，进行ＰＣＲ扩增，得到６５９ｂｐ在氟烷基因中（即兰尼定受体基因）中第１８４３个碱基的特异片段，经过ＨｈａⅠ酶切和电泳来检测氟烷基因的变异，即其基因型。结果表明：（１）５６头长白猪中，阳性猪（ｎ／ｎ）１头占１·７９％，杂合子猪（Ｎ／ｎ）为１８头，占３２·１４％，阴性猪（Ｎ／Ｎ）为３７头，占６６·０７％。杂合子数实际值显著高于理论值，说明杂合子猪具有杂种优势，因而易被选作种用。Ｈａｌ￣N基因频率０．８２，Ｈａｌ￣ｎ的基因频率为０·１８。（２）每头猪采集２～４根含毛囊的毛样，就可进行ＰＣＲ扩增，采样方便且一次可大批量进行。（３）从１周龄仔猪开始至成年的种猪，均可用毛样进行基围诊断。
Hairs plucked form 56 Denmark landrace(17-day-old piglet,26 male breedingPigs and 29 female breeding pigs) were used for DNA extraction and PCR amplifica-tion.The specific 659 bp containing the mutation point in ryanodine receptor gene wereamplified effectively.The genotypes for halothane were diagnosed by digestion of Hha Irestriction endonulease and electrophoresis.The results showed as follows,1.It was indicated that there were one halothane positive pig(genotype nn),18heterozygous pigs (genotype Nn)and 37 halothane nagative pigs(genotype NN)amongthe pigs examined.the frequency of the different genotypes were 1.79%,32.14%and66.07%respectively,gene frequency of Hal and Hal were0.82 and0.18 respectivly. 2. The technique adopted in this test only requires four hairs of each pigs forPCR amplification and gene diagnosis.It certainly takes advantages over other methodsfor its simplicity,rapidity,sensitivity and specificity.3.Hair sample can be taken from the pigs of 7 days or over and used for diagno-sis of the genotypes for halothane.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1996年第2期137-141,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省科委课题
关键词
应激综合征
氟烷基因
PCR
猪毛
猪病
Porcie stress syndrome,Halothane gene,PCR amplification Pluckedhair
