摘要
目的:通过对天然药物丹参酮A对增生性瘢痕成纤维细胞凋亡作用的研究,以期寻找新的治疗病理性瘢痕的有效药物。方法:以四甲基偶氮唑(MTT)法、流式细胞术(FCM)和特异性荧光染色法检测丹参酮A对成纤维细胞增殖和凋亡的影响。结果:丹参酮A对成纤维细胞的增殖具有显著的抑制作用,且呈现浓度和时间依赖性。采用流式细胞术和特异性荧光染色法检测凋亡指数,均呈剂量依赖性增高(P<0.01)。结论:丹参酮A对瘢痕成纤维细胞的增殖具有显著的抑制作用,并且能够诱导其发生凋亡。
Objective： To observe the effect of herbal medicine Tan ⅡA on proliferation and apoptosis of scar-derived fibroblasts and to screen new drug for treatment of human pathological sear. Methods： The fibroblasts were cultured with Tan ⅡA and then the cell growth rate was determined with MTT assay and the apoptosis index was analyzed with flow eytometry （FCM） and fluorescent staining. Results： Compared with the control group, the fibroblasts significantly reduced their growth rate （according to MTT assay） and inerease their apoptosis index （according to FCM and fluorescent staining measurement） after exposed to Tan ⅡA in a concentration and time-de- pendent manner（P〈0. 01）. Conclusions： Tan ⅡA could inhibit proliferation and induce apoptosis of cultured fibroblasts.
出处
《广东医学院学报》
2005年第4期359-360,共2页
Journal of Guangdong Medical College
基金
广东省自然科学基金资助项目(编号036736)
广东省中医药局科研课题资助项目(编号103074)
湛江市2003年科技招标资助项目
关键词
丹参酮ⅡA
成纤维细胞
凋亡
Tan ⅡA
fibroblasts
proliferation
apoptosis
