摘要
本文主要从酵母载体系统的选择、酵母表达宿主的选择、多拷贝菌株的构建、高密度发酵培养酵母细胞、应用酵母系统表达外源基因的研究现状、酵母表达系统的缺陷及对策等几个方面综述了近年来酵母表达体系在基因工程重组蛋白药物开发方面的研究进展。
This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering, such as the selection of yeast vector system and yeast expression strain, construction of multi-copy strain, the high yeast cell density culture, the recent situation of yeast system applied in expression gene, defect of yeast expression system and countermeasure,etc.
出处
《化学工业与工程》
CAS
2005年第5期381-385,共5页
Chemical Industry and Engineering
关键词
酵母表达体系
基因工程
重组蛋白药物
yeast expression system
gene engineering
recombinant protein drug
