摘要
以黑曲霉(Aspergillusniger)为出发菌株,经紫外线(UV)、硫酸二乙酯(DES)、60Co诱变,筛选出β-葡萄糖苷酶的活力比原菌株明显提高。并通过单因素和正交实验研究了该酶的固体发酵条件,采用优化配方,使酶活力达到169.2U/ml。
The Aspergillus niger mutant strain was been selected by alternative treating Aspergillus niger with UV, EDS and ^60Co. The enzyme activity of β-glucosidase from fermentation of the mutant was heightened compared with the original. Further more the fermentation conditions for the mutant were optimized through single factor and orthogonal experiments. Under the optimal condition, the enzyme activity of β-glueosidase attained 169.2 U/ml.
出处
《安徽农业科学》
CAS
北大核心
2005年第8期1566-1568,共3页
Journal of Anhui Agricultural Sciences
关键词
黑曲霉
Β-葡萄糖苷酶
诱变
固体发酵
Aspergillus niger
β-glucosidase
Solid state fermentation
Mutation
