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RT-PCR检测bFGF对脊髓损伤后bcl-2、bax基因的表达 被引量:2

The Effect of Basic Fibroblast Growth Factor on the Expression of bcl-2 and bax Gene in Spinal Cord Injury
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摘要 目的研究碱性成纤维细胞生长因子(bFGF)对脊髓损伤后bcl2和bax基因表达的影响。方法利用AllenWD法以25gcf致伤SD大鼠脊髓制作损伤模型,经蛛网膜下腔导管于术后即刻、0.5,1,2,3,4,6,12,24,48h导管注入bFGF20μL(含bFGF200U);对照组相同时间点注入等量生理盐水作对照。术后2,6,12,24,48h取损伤脊髓组织,通过逆转录PCR(RTPCR)检测bcl2、bax基因的表达。结果脊髓损伤后应用bFGF显著促进bcl2基因的表达,抑制bax基因表达,数据差异有显著性。结论bFGF通过促进bcl2基因的表达、抑制bax基因的表达抑制脊髓损伤后神经细胞的凋亡,从而保护脊髓组织,这可能是bFGF对脊髓损伤具有保护作用的机制之一。 Objective To investigate the temporal effect of basic fibroblast growth factor(bFGF) on the expression of bcl-2 and bax gene in the injured spinal cord of rats. Methods 60 Sprague-Dawley rats received 25 gcf impact to form Ts spinalcord injure (SCI) were divided by random into two groups, and a thin plastic tube inserted into subarachnoid space for perfusion of bFGF. bFGF-treated animals received bFGF 200 U(20μL liquid) at 0, 0.5, 1, 2, 3, 4, 6, 12, 24 and 48 hours after injury. The saline-treated animals received the equal volume of normal saline as contrt. The rats were sacrificed at 2, 6, 12, 24 and 48 hours afterinjury( n = 6). The tissues in the injured cord were collected and performed sections. The sections were tested by RT-PCR for the bcl-2, bax gene. Results The levels of bcl-2 mRNA in bFGF-treated animals after SCI were increased strikingly than that in control(0. 406 vs 0. 296 at 2 h after ingury, 0. 332 vs 0.26 at 48 h after injury, P〈0.01). However, the levels of bax mRNA of bFGF-treated animals after SCI were reduced strikingly than in control(0. 505 vs 0. 636 at 2 h after ingury, 0. 436 vs 0. 588 at 48 h after injury P 〈 0.01). Conclusion bFGF can enhance the expression of bcl-2 gene and attenuate the expression of bax gene, this means that bFGF can inhibit apoptosis after spinal cord injury.
出处 《福建医科大学学报》 2005年第3期281-283,共3页 Journal of Fujian Medical University
基金 福建省自然科学基金资助项目(F0110004) 福建省卫生厅青年基金资助项目(2001110)
关键词 脊髓损伤 成纤维细胞生长因子 碱性 脱噬作用 基因 BCL-2 原癌基因蛋白质类 spinal cord injury basic fibroblast growth factor apoptosis genes, bcl-2 proto-oncogene proteins
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