摘要
目的研究腺病毒载体介导的IL-3基因疗法对放射损伤小鼠造血功能重建的影响.方法将构建的IL-3腺病毒(AdCMVIL-3)经腹腔注射至5Gyγ射线放射损伤小鼠体内,并与注射空载体(AdCMVNull)相对照,检测外周血WBC、Hb及PLT的动态变化.结果空载体注射组于照射后产生严重的全血细胞下降:PLT于照后6～12d降至200×109/L以下,WBC于照后6～15d降至1.0×109/L以下,Hb于照后12～15d降至60/L以下.AdCMVIL-3治疗组,WBC下降的程度明显减轻,其最低值高出对照组近2倍,而且WBC的恢复时间较对照组提前约17d.同时显示有一定程度的促进PLT和Hb恢复的作用.结论腺病毒载体介导的IL-3基因疗法可显著促进放射损伤小鼠WBC的恢复,对Hb、PLT的恢复也具有一定的促进作用.
Objectlve To study the lnterleukin-3 gene therapy mediated by recombinant adenovirus vector on hemopoietic reconstitution of mice injured by irradiation. Methods Replication-deficient mouse interleukin-3 （IL-3） cDNA recombinant adenoviruses （AdCMVIL-3） was injected through intraperitoneal in the mice injured by 5. 0Gy γirradiation, taken AdCMVNull as control. WBC, Hb and PLT were examined during the course. Results In animals receiving the AdCMVNull, the radiation induced a profound pancytopenia with the nadir of WBC bellow 1.0 × 10^9/L from 6d to 15d, Hb bellow 60/L from 12d to 15d, and PLT bellow 200 × 10^9/L from 6d to 12d. When AdCMVIL-3 was administered immediately after irradiation, the WBC levels were sustained, with a nadir almost 2 fold higher than that of control, and the recovery to normal levels and above was accelerated by more than two weeks. Meanwhile,there was a trend towards accelerated recovery of the PLT as well as the Hb. Conclusion IL-3 gene therapy could mainly accelerate the recovery of WBC and had some extent effect on the recovery of PLT and Hb.
出处
《重庆医学》
CAS
CSCD
2005年第9期1290-1292,共3页
Chongqing medicine
基金
重庆市应用基础研究基金资助课题(2002)
