摘要
目的探讨中药汉防已甲素、罗通定及川芎嗪逆转肿瘤细胞株KBV200多药耐药性作用。方法用四唑盐比色法检测细胞株对药物敏感性,用逆转录多聚酶链反应检测耐药株用药前后肿瘤多药耐药基因的表达。结果在最适逆转浓度下,汉防已甲素(10-6mol/L),罗通定(10-5mol/L)对KBV200耐药性有明显逆转作用,川芎嗪(10-4mol/L)有一定作用,使长春新碱对耐药细胞株KBV200半数致死量由1 122nmol/L分别降至32 nmol/L、35.8nmol/L及541nmol/L。在上述浓度下,3种逆转剂分别与长春新碱(400nmol/L)合用,细胞存活率分别为0.32±0.1、0.29±0.05、0.56±0.1,较对照0.76±0.05有显著差异(P<0.001)。在同样浓度下,3种逆转剂都能使耐药株肿瘤多药耐药基因mRNA表达降低,但与对照比,无显著性差异。结论汉防已甲素、罗通定逆转肿瘤多药耐药性效果好,川芎嗪有一定逆转作用,它们不良反应小,有良好临床应用前景。
Objective To investigate the reversal effect of three Chinese medicine tetrandrae（TTD） ,rotundini（RTD） and ligustrazini（LTZ） on human muhidrug resistant KBV200 cell line. Methods The chemosensitivity of KBV200 was detected with MTT assay in vitro and the level of MDR1mRNA of KBV200 after TTD, RTD, LTZ treatment was examined with RT-PCR. Results With the concentration 10^-6 mol/L of TTD, 10^-5 mol/L of RTD, and 10^-4 mol/L of LTZ, these Chinese medicine could increase VCR cytotoxicity on KBV 200 and the 50% inhibitory concentration（IC50） of VCR on KBV200 decreased from 1122nmol/L to 32 nmol/L, 35.8 nmol/L and 541 nmol/L, respectively. The combination of TTD, RTD, LTZ and VCR（400nmol/L） respectively decreased cell surviving fraction from 0.76±0.05 to 0. 32±0. 06, 0.29±0.05 and 0.56±0.01（P〈0. 001-0.01）. The expression of MDR1mRNA of KBV200 decreased after treatment of these Chinese medicine with the above concentration. But the difference is not significant compared with the control. Conclusion These data indicated that TTD, RTD, LTZ could reverse VCR resistance of KBV200. They may be useful in enhancing the clinical effectiveness of VCR.
出处
《重庆医学》
CAS
CSCD
2005年第9期1383-1384,1386,共3页
Chongqing medicine
关键词
汉防已甲素
罗通定
川芎嗪
多药耐药性
逆转录多聚酶链反应
tetrandrae
rotundini
ligustrazini
multidrug resistance
reverse transcription polymerase chain reaction
