摘要
目的:研究氨硝西泮在大鼠血清及脑组织药物浓度的动态变化。方法:采用高效液相色谱法(HPLC)测定药物浓度,色谱柱为C18柱(200mm×4.6mm,4μm),流动相为甲醇∶水(55∶45),流速:0.8ml/min,检测波长310nm,柱温25℃。对48只健康成年SD大鼠血清及脑匀浆上清液经氯仿提取,挥干,用流动相重溶,进行色谱分析。结果:单次给药后大鼠血清及脑组织药物浓度快速下降,尤其是脑组织;重复给药后,血清及脑组织药物浓度再次升高。结论:氯硝西泮血清及脑组织中药物浓度的快速下降,可以导致惊厥复发。氯硝西泮重复给药可有效地控制惊厥复发,但也可引起药物在体内的蓄积。
Objective ： To study the dynamic change of clonazepam＇ s concentration in the serum and brain of rat. Methods： High pressure liquid chromatography was used to detect the concentration of clonazepam, with Kromasil C18 column as stationary phase, methanol-water （55 ： 45） as mobile phase with the flow rate of 0. 8ml/min. The temperature of column was 25℃; the wavelength of detection was 310nm. Clonazepam was extracted from serum and brain of forty-eight healthy SD rats by chloroform and solved in mobile phase. Results： In single dose group, the concentration of CZP decreased rapidly, especially in brain. Repeated administration of CZP at 30 min maintained a high concentration in serum and brain for a relatively long time. Conclusion ： The rapid decrease of the concentration of clonazepam was the reason for seizure relapse. Repeated administration of CZP would avoid this, but might lead to drug accumulation in the body.
出处
《陕西医学杂志》
CAS
北大核心
2005年第9期1043-1045,共3页
Shaanxi Medical Journal
